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Image Search Results
Journal: Science immunology
Article Title: Human Innate Lymphoid Cell Precursors Express CD48 that Modulates ILC Differentiation through 2B4 Signaling
doi: 10.1126/sciimmunol.aay4218
Figure Lengend Snippet: (A) Freshly isolated UCB-derived CD34+ HSCs were stained for CD244 and CD48 (representative, n=4). (B) CD244 in CD34+CD48− cells (aqua) and in CD34+CD48+ cells (red) for day 1 and day 3 UCB-derived CD34+ HSCs (representative, n=4). (C) SAP mRNA expression in day 5 CD34+CD48− and CD34+CD48+ cells (n=4/group). (D and E) Anti-CD244 and anti-CD48 blocking antibodies or isotype IgG controls? were added to differentiating CD34+α4β7+CD48+ cultures to block CD244 signaling. (F and G) CD34+α4β7+CD48+ cells were plated on anti-CD244 or IgG coated plates twice for 4 days prior to 21 days of differentiation to activate CD244 signaling via cross-linking. Cells were stained for ILCs, and the percentage (D and F) or absolute number (E and G) of CD94+ NK cells, CD294+ ILC2 and CD117+ ILC3 are shown (n=4/group). (H) CD244+ cultures are shown for cells transfected with control or CD244 gRNA (n=3/group). (I) Representative ILC2 (CD294) staining for cultures transfected with control or CD244 gRNA. (J) Dot plot showing generation of ILC2s from cultures that express (control gRNA) or lack (CD244 gRNA) CD244. (C-H) Mean ± SD, paired t-tests (C, F, G and H) or one-way ANOVA (D and E) and p-values are depicted.
Article Snippet: The Alt-R Cas9 Nuclease V3, universal tracrRNA, CD244 gRNA and
Techniques: Isolation, Derivative Assay, Staining, Expressing, Blocking Assay, Transfection
Journal: BMC Genetics
Article Title: Copy number variation of CCL3L1 among three major ethnic groups in Malaysia
doi: 10.1186/s12863-019-0803-3
Figure Lengend Snippet: Distribution of CCL3L1 copy number values among three major ethnic groups in Malaysia and Europe. A significant difference was observed in which the Malay and Indian showed a copy number range from 0 to 8, the Chinese copy number range was 0 to 10 while the Europeans have a copy number range of between 0 and 4
Article Snippet:
Techniques: